导入AGPase基因的转基因可育水稻及其经济性状的研究

以农杆菌介导法将淀粉合成关键酶AGPase(腺嘌呤二磷酸葡萄糖焦磷酸化酶）基因导入云南地方优质水稻合系35，38，39，41，42中，获得了大量的可育转基因植株。PCR扩增检测，外源基因已稳定整合进水稻基因组中。大量的农艺性状及淀粉含量研究表明，转基因水稻种子的千粒重比对照明显提高，但总淀粉含量没有显著变化。     

硝酸银在水稻农杆菌转化中的作用

以籼稻、粳稻两种类型的成熟种子为外植体研究了不同浓度的AgNO3在水稻农杆菌转化中的作用。结果表明，在农杆菌转化前添加AgNO3可以提高愈伤组织诱导率，最适浓度为8～10mg／L，但对愈伤组织的分化没有明显促进作用；转化后添加低浓度的AgNO3可以减轻愈伤组织的褐化，提高抗性愈伤率和绿苗分化率，最适浓度为2-8mg／L。同时发现，无论是转化前还是转化后，添加高浓度的AgNO3对水稻愈伤组织的分化均具有毒害作用。     

中国葡萄野生种对葡萄根癌病的抗性

通过田间接种鉴定，系统研究了中国葡萄属１３个种和变种的２６个株系对葡萄根癌病的抗性，筛选出高抗株系１０个。其中燕山葡萄（♀）因扦插容易生根，可用自根苗作为抗葡萄根癌病砧木进行嫁接试验，其余可用作抗葡萄根癌病的育种资源     

Chromosome and plasmid-encoded N-acyl homoserine lactones produced by Agrobacterium vitis wildtype and mutants that differ in their interactions with grape and tobacco

Agrobacterium vitis causes crown gall disease on grapevines. It also induces a specific necrosis on grape roots and a hypersensitive response (HR) on tobacco that are regulated by a complex quorum-sensing regulatory system. Strain F2/5 produces at least six N-acyl-homoserine lactones (AHLs) that function as signal molecules in quorum-sensing. The AHLs differ in acyl side chain length (8–16 carbons) as determined by gas chromatography/mass spectrometry and electrospray ionization tandem mass spectrometry. Mutant derivatives of F2/5 differ in ability to cause necrosis and the HR and show variable AHL profiles as determined by a thin-layer chromatography/biosensor assay. All wildtype A. vitis strains revealed the presence of long-chain AHLs regardless of tumorigenicity or ability to cause the HR. Whereas genes encoding long-chain AHLs are predicted to reside on the F2/5 chromosome, the determinants for short-chain AHLs were shown to be located on conjugal plasmids.     

根癌农杆菌介导绿色荧光蛋白基因转化印度酸桔的研究

 通过根癌农杆菌介导将绿色荧光蛋白基因转入印度酸桔的胚性愈伤组织中, 经潮霉素筛选,获得抗性愈伤组织, 并再生植株。对这些植株进行GUS 染色、PCR 分析、绿色荧光检测和Sourthern 杂交验证, 结果表明绿色荧光蛋白已经在转基因植株中表达。     

桃树根癌病防治技术探讨

桃树根癌病是公认最难防治的土传细菌病害之一。在对该病进行较系统研究的基础上,采用多种抗菌药剂、多种方法进行了室内、外药敏测定与防治试验,显示出异菌氰、次氯酸钠、乙蒜素、妥布霉素、环丙沙星、庆大霉素及其混用对防治桃树根癌病菌有明显防效。     

Chromosomal and Plasmid Diversity of Agrobacterium Strains Isolated from Ficus benjamina Tumors

Agrobacteria were previously isolated from tumors developing on branches and aerial and hypogeous roots of weeping fig plants in Italy and in The Netherlands. A representative group of 48 strains was analyzed by PCR–RFLP of 16S and 16S + IGS ribosomal regions, PCR–RFLP of six Ti plasmid (pTi) regions and characterized for plasmid content. Two groups of agrobacteria were separated by cluster analysis of PCR–RFLP profiles of rrs gene: seventeen strains were similar to the new species Agrobacterium larrymoorei, while the remaining strains were included within the agrobacterium biovar 1 group. Sixteen different plasmid profiles from one to five plasmids were observed. In addition, 21 ribotypes and 20 pTi structures were arranged in many different combinations, showing that fig agrobacteria were characterized by a wide heterogeneity. A general lack of correlation between strain ribotypes and plasmid content was observed.     

农杆菌介导遗传转化在水稻基因工程育种中的应用

随着生物技术的发展，水稻基因工程育种在水稻育种中发挥着越来越重要的作用。在诸多的转基因方法中，农杆菌作为一种天然的植物基因转化系统，以其独特优点而倍受重视。本文概述了农杆菌介导转化水稻的原理，农杆菌介导转化水稻的优点并且介绍了农杆菌菌株、受体材料和培养基成分的不同对农杆菌介导转化水稻的影响。另外对农杆菌介导遗传转化在水稻抗虫基因工程育种、抗病基因工程育种、抗逆基因工程育种、优质基因工程育种、耐除草剂基因工程育种及提高水稻光合效率和延缓衰老方面的研究进展进行了综述。最后对农杆菌介导遗传转化在水稻基因工程育种中存在的问题和发展前景做了简要说明。